Overview of the Core
The Purdue Institute of Integrative Neuroscience (PIIN) Cell Engineering Core was established to facilitate stem-cell-related research for the Purdue community by providing induced pluripotent stem cell (iPSC) lines and multiple differentiated cell types from human iPSCs, with a special focus on neuronal cells. The Core uses cutting-edge technologies to induce and differentiate iPSCs for studying disease mechanisms in-a-dish, drug screening and other applications.
• iPSC derivation using non-integrating methologies (e.g. mRNA, Sendai virus, episomal vector) from human skin biopsy, peripheral/cord blood, endothelial progenitor cells (EPCs) and urine epithelial cells
• iPSC expansion, distribution and differentiation
• Cell Repository: iPSCs, fibroblasts, peripheral blood monocytes (PBMs), EPCs, urine epithelial cells, neural precursors, cardiomyocytes and other cell types
• Functional studies: Microelectrode array (MEA) using Maestro microelectrode array from Axion Biosystems
• Training & consultation for iPSC culture, reprogramming and differentiation
Jungi-Il Moon, Ph.D. Core Coordinator