The Cell Programming Core Facility (CPCF) at the University of Bonn builds on the expertise of the Institute of Reconstructive Neurobiology in cellular reprogramming, genome engineering, and stem cell differentiation. Our mission is to support researchers in establishing advanced cellular disease models - including organoids - derived from patient-specific stem cells.
As starting material, we accept skin fibroblasts or peripheral blood mononuclear cells for the generation of iPS cells. Upon request, selected iPS clones undergo standardized quality control, including pluripotency assessment, transgene clearance, genetic fingerprinting, and SNP array–based karyotyping.
The CPCF also provides genome editing services, enabling the creation of isogenic iPS lines with knockout alleles, point mutations, knock-ins, or targeted integration of expression constructs at the AAVS1 safe harbor locus. In addition, the facility offers broad expertise in iPS cell differentiation, including the generation of neural precursors, terminally differentiated neurons, microglia, spheroids, and organoids. Currently, we provide stable neural progenitor cells (NPCs), cortical NPCs, and NGN2-induced excitatory forebrain neurons, all available in cryopreserved formats.
Michael Peitz, PhD
Cell Programming
Institute of Reconstructive Neurobiology
University Hospital of Bonn
