Gene Editing Interest Group-Pipelines for single-nucleotide editing and discussion on what is the proper isogenic control.
Reprogramming Interest Group-Reprogramming with Sendai virus method and testing Sev elimination
Differentiation Interest Group- Generation of skeletal muscle cells from iPSCs and comparison of in-house and commercial motor neuron differentiation media.
Gene Editing Interest Group-DNA repair template and related strategies/obstacles in generating CRISPR-based knockins
Reprogramming Interest Group-iPSC reprogramming using mRNA
Differentiation Interest Group-Generating neurons via inducible NGN-2 expression in iPSCs
Gene Editing Interest Group (joint with CoEuStem)-Pipelines for assessing genomic integrity using SNP arrays (karyotyping with SNP arrays)
Spotlight presentation: Giovanni Faga & Jacopo Zasso presented the work at the Human Technopole in Milan, Italy
Spotlight presentation: Jose Polo, Director of Adelaide Centre for Epigenetics at the School of Biomedicine, University of Adelaide, Australia gave a presentation on TNT reprogramming “Transient naive reprogramming corrects hiPS cells functionally and epigenetically”.
Spotlight presentation: Laurie Lacko, the manager director of the Weill Cornell Human Therapeutic Organoid Core gave a presentation on her new core and pancreatic islet organoids.
Spotlight presentation: Steven Watters, Director North America Sales, High Res BioSolutions, gave a presentation about automation titled “Automation that makes life better”.
Dan Cortes, head of the stem cell core lab at HHMI/Janelia, joined COREdinates and briefly introduced himself to the group.
Spotlight presentation
Bill Skarnes from Jax gave a presentation on “SNP array analysis of iPSC sublines reveals a high incidence of novel copy number variation”.
Gene editing group, interactive discussion
Spotlight presentation: An overview of the gene-editing pipeline at the Children’s Hospital of Philadelphia (CHOP) Stem Cell/Gene Editing Core, led by Dr. Giulia Pavani (Core Director).
Open Q&A and discussion: questions on CRISPR design/validation, editing in iPSCs and primary cells, delivery methods, off-target assessment, QC, workflows, costing/throughput, and training/QA practices.
Spotlight presentation: Evan Graham, Business Development Manager and Head of Outreach and Learning at BioLamina gave a presentation on “ Practicality, Pluripotency, Predictability, and Precision: Introducing the BioLamina Core Facility Initiative”.
Sebastian Diecke, PhD, Max-Delbrück-Centrum für Molekulare Medizin (MDC)
“Functional pluripotency assessment – approach groups “
Troubleshooting clinic
“New Reprogramming methods pro and cons of high efficiency mRNA repro, discussion on single clones vs bulk”
“Closing the user-to-designer Loop: Using Stem Cell and Organoid Insights to Guide AI-Driven Protein Engineering”
