CHOP Pluripotent Stem Cell Core Facility
The CHOP Pluripotent Stem Cell Core was established in 2008 as part of the Center for Molecular and Cellular Therapeutics, serving the research needs of the scientific community in the field of human pluripotent stem cell (PSC) biology. We have created an infrastructure at CHOP to generate induced pluripotent stem cell (iPSC) lines from human patients for long-term studies on multiple diseases. Using iPSC lines derived by the core facility, we have built a solid foundation for studying pediatric diseases with a special emphasis on benign hematological disorders. Our multi-investigator driven studies have established rigorous parameters for analyzing normal and pathological iPSC-derived hematopoiesis in defined pediatric disorders. We and others have shown that various hematopoietic pathologies can be recapitulated through in vitro differentiation of patient-derived iPSCs. We provide enrichment courses to actively train investigators with the necessary tools and expertise to maximize successful outcomes. We operate on a fee-for-service basis to the global research community using cutting edge technologies. Stringent protocols have been established to:
1. Generate iPSCs from human cells using non-integrating reprogramming systems
2. Differentiate human PSCs into the various germ layers and their derivative tissues
3. Propagate and distribute human PSC lines to investigators
4. Provide tested, quality-controlled cell culture reagents to investigators
5. Perform genome editing on PSC lines for mutation and transgene expression analyses
Contact:
Deborah L. French, PhD
Director, CHOP Pluripotent Stem Cell Core
3501 Civic Center Blvd, CTRB 5014
Philadelphia, PA 19104
267-426-9673
frenchd@email.chop.edu
Services offered:
Cell derivation and characterization
Differentiation
Genome editing
Cell line distribution
Enrichment courses